It's OK if purity isn't great, since you'll get separation of cell populations in the scRNA-seq Bead-based purification of cells is preferred over FACS, because it is shorter and usually results in happier cells.This is be fine for flow cytometry, but may damage cells and cause problems for scRNA-seq. bashing up lymph nodes with a rubber policeman). You can purchase ACK here, or make your own using the recipe below: ![]() Avoid long incubations or RT incubations with this buffer. ![]() If there are still visible RBCs present after pelleting the cells, you should repeat the lysis step.
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